Effects associated with insertion of rol genes on morphogenic potential in explants derived from transgenic Bacopa monnieri (L.) Wettst

The present study deals with the establishment of rolA-transgenic and rolB-transgenic plants for first time through Agrobacterium tumefaciens mediated transformation, exploiting inherent morphogenic potential an important medicinal plant, Bacopa monnieri (L.) Wettst. showed integration expression rolA rolB genes respectively, whereas Ri-transformed rolA, rolB, rolC rolD genes. Morphogenic different types explants derived from rolA-transgenic, on basal medium was evaluated. Shoot organogenesis enhanced significantly in leaf (1.6-fold) internode (1.4-fold) plants, (2.4-fold) as well (5.2-fold) (3.3-fold) compared to non-transformed plants. Substantial increase root also noticed (1.7-fold) explants, (3.6-fold) (4.1-fold) (1.9-fold) ones. In addition this, growth tip shoot regeneration but not roots. Clones each transgenic plant line harboring rol depicted notable phenotypic changes including reduced length, increased number nodes/plant, leaves/plant roots/plant. morphology altered Insertion genes, individual or combination, rhizogenes resulted excised alteration phenotype maintained vitro.